利申定先小薯一隻 唔少野都係supervisor度聽返黎
唔好盡信我 都唔好盡信乜乜倒regenerative med clinic
呢個field水好深 因為好多therapy都係claim緊呢種"stem cell"可以有乜乜乜功效從而用黎賺錢
但係好多都未有direct proof
不過business 角度真心唔熟 不予置評
係research角度其實都幾亂下
一大原因就係"mesenchymal stem cell"呢個term 以及"msc"呢個簡稱用得好差
宜家一般共識係佢未算stem cell (lack of proof of long term self renewal)
更大問題連"what is msc"都係各有各講
唔同studies隨時睇緊唔同嘅population
可能用緊cells from different organ of origin (bone marrow/adipose tissue/umbilical cord/muscle/etc)
有唔同嘅isolation strategies
但係全部都話自己係msc 唔亂就奇
Still msc is known to be multipotent progenitor(我supervisor 立場係叫佢做multipotent/mesenchymal stromal cell)
即係可以變adipocyte/fibroblast/osteocyte/chondrocyte
(btw fibroblast in this context≈scar forming cell)
但係真係打落關節後佢會變咩就要睇兩樣野
一係intrinsic factor
即係個cell本身會唔會already partly committed to adipogenesis/fibrogenesis/etc?
近年single cell rna sequencing in msc就搵到唔同subpopulations
簡單咁講即係其實有msc "type"1/2/3/4/...
可能type 1就係特別鍾意變adipocytes
type 2特別鍾意變osteocytes (9up example only)
咁你打左呢幾種落關節仲唔瀨野?
另外就要睇local environment (ie "niche")
附近嘅cell produce左咩factors 甚至係physical property (elastic modulus/viscoelasticity)都會影響到cell fate commitment
特別係傷緊發炎緊嘅會只會更加亂
假設真係有某msc subtype within svf can specifically give rise to chondrocyte
單靠centrifugation physically isolate fat-derived msc
無select against唔關事嘅cell 又無enrich你真正需要個population
實際效果有幾多呢
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