Base Excision Repair(BER):
1. DNA glycosylases cleave the N-glycosidic bond and generate AP sites (Abasic sites). Different DNA glycosylases are used to remove different damaged bases.
2. AP endonucleases cleave the DNA at the site of damage.
3. dRpase (Deoxyribophosphodiesterase) removes several sugar-phosphate residue.
4. DNA polymerase inserts new stretch of DNA and the gap is sealed by DNA ligase.
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Nucleotide Excision Repair (NER):
- It repairs a range of DNA damage while BER repairs lesions for specific DNA glycosylase.
1. The damaged strand is cleaved on either side of the side of lesions.
2. The damaged single strand DNA is displaced.
3. DNA polymerase synthesizes new DNA to fill the gap and seals the nick by DNA ligase
- It repairs a range of DNA damage while BER repairs lesions for specific DNA glycosylase.
1. The damaged strand is cleaved on either side of the side of lesions.
2. The damaged single strand DNA is displaced.
3. DNA polymerase synthesizes new DNA to fill the gap and seals the nick by DNA ligase
revising
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